Research

Aims:

Our goal is to understand and manipulate IRBs between closely related species in Solanum. In the first phase of this project, we identified key pollen and pistil IRB genes in the tomato clade, selected informative germplasm for mechanistic and evolutionary studies, and manipulated IRBs from both the pollen- and pistil-side. In this phase, we propose to:

Data & Tools

To access public data please read the IRB Tomato Data Release Agreement

Publicly available data include...

iTRAQ Proteomics - Style Development

iTRAQ Proteomics - pollen from cultivar and S. pennellii

454 Pollen and Style Transcriptomes

FISH Cassette for Mutagenesis - pFCM plasmid map

IRB Tomato and its members are pleased to share data and resources that may strengthen and enable research within the scientific community by making these resources available prior to publication with the expectation that the community will not encroach on our efforts to publish whole scale analysis based on this data. We invite members of the community to contact us regarding these data assemblies because they have not been thoroughly examined or reviewed and thus may contain errors and should be considered preliminary. The use should verify or validate any sequence that they suspect may be in error.

Thus, by accepting the terms of this agreement and accessing the associated data, you are agreeing not to publish whole scale analyses of the data sets including the draft iTRAQ Solanum species pollen and style proteome or draft 454 Solanum species pollen and style transcript analyses of protein/gene families/genome structures/repetitive sequences, comparative analyses with other organisms including other genomes, or other analyses such that they encroach and limit our opportunity to publish the findings of our work.

The Fish Cassette for Mutagenesis construct, pFCM100, may be requested by contacting us directly. Due to limitation in pFCM100 quantity, stock will be distributed to those demonstrating a sound scientific request..

The data may be downloaded and used by those who agree to these terms of this release. It should not be further re-distributed or publicly released. If the data are used in a publication, it should be cited as:

• “iTRAQ pollen and style Proteome sequence data was obtained from the Interspecific Reproductive Barriers in Tomato research group funded by the National Science Foundation”
• “454 pollen and style transcript sequence data was obtained from the Interspecific Reproductive Barriers in Tomato research group funded by the National Science Foundation”

or

• “Fish Cassette for Mutagenesis, pFCM100, was obtained from the Interspecific Reproductive Barriers in Tomato research group funded by the National Science Foundation”.